Dagdelen Serife
Analytical and Inorganic Chemistry
Laboratory of Theory and Applications of Electrodes
Thermoresponsive hybrid microgel modified with supermaramagnetic iron oxide nanoparticles as degradable carrier for magnetic hyperthermia controlled drug release
Serife Dagdelen
Supervisor: dr hab. Marcin Karbarz
Co-supervisor: dr Marcin Mackiewicz
Novel degradable, hybrid nanogel as hyperthermia drug carrier based on poly(N- isopropylacrylamide) (pNIPA) cross-linked with N,N’-bisacryloylcystine (BISS) and containing superparamagnetic iron oxide nanoparticles (SPION@CA) were synthesized by semi-batch precipitation polymerization. pNIPA gave the nanogel sensitivity to temperature and BISS was responsible for degradation in presence of glutathione (GSH) (-S-S- bridges reductor existed in cells), while SPION@CA allowed to control temperature remotely to improve releasing of drug releasing. Microgels exhibited volume phase transition temperature at ca. 34°C which is near the body temperature and were stable at high ionic strength. It was demonstrated that presence of SPION@CA in the polymer network of the microgels enable increase temperature up to 42°C and had positive influence on enhanced releasing of anticancer drug – doxorubicin (DOX). The highest releasing of DOX was observed in condition typical for cancer cells: pH ca. 5, 42°C and presence of GSH. The lowest amount of DOX was released from the nanogel at pH 7.4 and absence of GSH. In vitro experiments proved that drug-free carrier was non-toxic against used cell lines, while the DOX-loaded microgels were more cytotoxic compared to free drug for MCF-7. The reported particle properties may open new possibilities in application of such materials in the fields of controlled release of various molecules and construction of sensors.
Literature:
[1] H. Farmanbordar, M.S. Amini-Fazl, R. Mohammadi, pH-Sensitive silica-based core-shell nanogel prepared via RAFT polymerization: investigation of the core size effect on the release profile of doxorubicin, New J Chem 45(46) (2021) 21824-21833.
[2] M. Mackiewicz, S. Dagdelen, K. Marcisz, E. Waleka-Bargiel, Z. Stojek, M. Karbarz, Redox-degradable microgel based on poly(acrylic acid) as drug-carrier with very high drug-loading capacity and decreased toxicity against healthy cells, Polym Degrad Stabil 190 (2021).
[3] X. Ling, J.S. Tu, J.Q. Wang, A. Shajii, N. Kong, C. Feng, Y. Zhang, M.K. Yu, T. Xie, Z. Bharwani, B.M. Aljaeid, B.Y. Shi, W. Tao, O.C. Farokhzad, Glutathione-Responsive Prodrug Nanoparticles for Effective Drug Delivery and Cancer Therapy, Acs Nano 13(1) (2019) 357-370.